What question did this study set out to answer?

To determine whether thermophilic Thermus thermophilus HB8 transaldolase can utilize nonphosphorylated aldoses to synthesize rare eight-carbon and nine-carbon higher sugars.

July 16, 2025Journal of Agricultural and Food Chemistry

Synthetic Study of 8- and 9-Carbon Sugars by Transaldolase

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To determine whether thermophilic Thermus thermophilus HB8 transaldolase can utilize nonphosphorylated aldoses to synthesize rare eight-carbon and nine-carbon higher sugars.
Assayed thermophilic Thermus thermophilus HB8 transaldolase with d-fructose-6-phosphate donor and various nonphosphorylated pentose and hexose acceptors.
Identified and verified higher-carbon reaction products using LC/MS and NMR spectroscopic analyses.
Solved the X-ray crystal structure of the transaldolase to evaluate the architecture of its catalytic acceptor binding pocket.
Transaldolase successfully synthesized eight-carbon octuloses using nonphosphorylated pentose acceptors: d-ribose (74% conversion), d-xylose (55%), l-arabinose (49%), and l-lyxose (84%).
The enzyme synthesized nine-carbon nonuloses using hexose acceptors: d-allose (13% conversion), d-galactose (56%), and l-altrose (71%).
X-ray crystallography revealed a wide and hydrophilic catalytic site that permits binding and reaction with nonphosphorylated aldose substrates.

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Implication

Biochemical study reveals synthesis of eight- and nine-carbon sugars using Thermus thermophilus transaldolase, indicating a viable enzymatic route to rare bioactive carbohydrates.

Cite This Study

Yoshihara et al. (2025) studied this question.

synapsesocial.com/papers/6a846b303dbf2f1b7f68c38b https://doi.org/10.1021/acs.jafc.5c05539

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Implication

Biochemical study reveals synthesis of eight- and nine-carbon sugars using Thermus thermophilus transaldolase, indicating a viable enzymatic route to rare bioactive carbohydrates.

Synthetic Study of 8- and 9-Carbon Sugars by Transaldolase

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Discussion

Implication

Cite This Study

Synthetic Study of 8- and 9-Carbon Sugars by Transaldolase

Key Points

Discussion

Implication

Cite This Study

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