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The production of an artifact by the methanolysis of total lipids of Ascaridia galli with boron trifluoride-methanol was dependent on the reaction time and the age of the reagent used. It was shown that this artifact was produced from oleic acid. Methanolysis of total lipids with sodium methoxide was complete in 10 min, and no artifact was produced.
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Fulk et al. (1970) studied this question.
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