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A simple fractionation method was developed to analyze phenolic compounds other than anthocyanins in red grapes using HPLC. The methanol extract of Concord and de Chaunac grapes was passed through a preconditioned C18 SEP-PAK and phenolic compounds other than anthocyanins were selectively fractionated with ethyl acetate. Anthocyanins were eluted with MeOH-HCl and analyzed by a spectrophotometric method. Phenolics other than anthocyanins were analyzed by HPLC. This technique is easy to use and convenient for phenolic analysis in both white and red grapes.
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Oszmiański et al. (1990) studied this question.
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