Expression Profiles of Differentially Expressed Circular RNAs and circRNA–miRNA–mRNA Regulatory Networks in SH-SY5Y Cells Infected with Coxsackievirus B5

Figures

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  Figure 1. Genomic features of circRNAs detected in CVB5-infected SH-SY5Y cells. (a) Venn diagram of the number of differentially expressed circRNAs in each group. A total of 2,366 circRNAs were expressed in two groups. (b) Genomic distribution of circRNAs. (c) Chromosomal distribution of circRNAs. (d) Length distribution statistics of circRNAs.

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  Figure 2. Differentially expressed circRNAs following CVB5 infection. (a) Box plots showing the expression levels of circRNAs from each sample. (b) Volcano plots depicting differentially expressed circRNAs following CVB5 infection. (c) Heatmap of maximally expressed circRNAs across all samples. (d) Venn diagrams of differentially expressed circRNAs identified in mock-infected and CVB5-infected SH-SY5Y cells.

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  Figure 3. Enrichment analyses of all differentially expressed circRNAs. (a) Biological process (BP), cell component (CC), and molecular factor (MF) of GO terms. (b)–(d) Directed acyclic graph (DAG) of BP, CC, and MF. (e) Top 20 KEGG pathways of host genes of all circular RNAs.

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  Figure 4. Validation of differentially expressed circRNAs by qRT-PCR. (a) Expression levels of six circRNAs validated by qRT-PCR. (b) Expression levels of six circRNA validated by RNA-seq.

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  Figure 5. Construction of the circRNA–miRNA–mRNA interaction network. (a) Network of circRNA hsa_circ_0008378. (b) Network of circRNA novel_circ_0014617.

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  Figure 6. Verification of circRNA hsa_circ_0008378 and novel_circ_0014617. (a) Western blot analysis of CVB5 specific protein VP1 expression transfected with pcDNA3.1-circRNAs. (b) RT-qPCR detected the expression of genes in IFN-I pathways (RIG-I, IRF3, MxA, OSAL, ISG15, and IFITM3). (c) RT-qPCR detected the expression of target miRNA according to the network. Data are representative of three independent experiments with similar results. Data are shown as mean ± SD (b) and (c). Ns, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001, two-tailed unpaired Student's t test.