What question did this study set out to answer?

To isolate and characterize the molecular structure, stability, and composition of aminopeptidase from autolyzed pig kidney tissue.

August 1, 1963Canadian Journal of Biochemistry and Physiology

A Conjugated Form of Aminopeptidase From Autolyzed Extracts of Kidney Tissue

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Authors

AMA. T. MathesonUniversity of Victoria SBS. BjerreUniversity of Toronto CHCharles S. HanesGoogle (United States)

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Implication

Biochemical analysis reveals that kidney aminopeptidase exists as a stable nucleic acid-conjugated complex, suggesting an endogenous mechanism for enzyme stabilization.

Key Points

To isolate and characterize the molecular structure, stability, and composition of aminopeptidase from autolyzed pig kidney tissue.
Prepared peptidase-rich fractions from autolyzed pig kidney extracts exposed to chloroform–octanol emulsification.
Purified and dissociated the enzyme complex using DEAE-cellulose chromatography and Sephadex gel filtration.
Assessed protein purity, charge, and activity using starch gel electrophoresis and ultraviolet absorbance (A280/260) spectrophotometry.
Kidney aminopeptidase occurred natively as a conjugated complex that dissociated under chromatography into an enzymatically active protein moiety and inactive nucleic acid-containing fractions.
The isolated protein moiety showed extreme lability upon dissociation and displayed an A280/260 absorbance ratio of 1.2.
Starch gel electrophoresis of the purified fraction resolved multiple enzymatically active protein bands alongside trace inactive protein.

Cite This Study

Matheson et al. (1963) studied this question.

synapsesocial.com/papers/6a84b30b192ab555d8a90cf6 https://doi.org/10.1139/o63-199

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Implication

Biochemical analysis reveals that kidney aminopeptidase exists as a stable nucleic acid-conjugated complex, suggesting an endogenous mechanism for enzyme stabilization.

A Conjugated Form of Aminopeptidase From Autolyzed Extracts of Kidney Tissue

Discussion

Member takes

Implication

Key Points

Cite This Study

A Conjugated Form of Aminopeptidase From Autolyzed Extracts of Kidney Tissue

Discussion

Member takes

Implication

Key Points

Cite This Study

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