Semen from bulls and boars was divided into three fractions and treated as follows: control, cold shocked, and frozen. There was a loss of lipid from bovine spermatozoa that had been cold shocked (P < 0.05) or frozen (P < 0.01) compared to the controls. This loss was acompanied by a significant increase in lipid in seminal plasma of the coI~'espouding' fractions
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Pickett et al. (1967) studied this question.
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