This paper continues the investigation of the assay and spectral properties of biological stains. The phenyl methane dyes, auramine O, basic and acid fuchsins, methyl violet, crystal violet, methyl green, and anilin blue W. S., may all be assayed by the spectrophotometric method. Of these, methyl green has been found unstable both in solid form and in solution, hence color densities of this dye must be measured promptly after preparation.
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Emery et al. (1953) studied this question.
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