What question did this study set out to answer?

To determine postmortem protein synthesis and degradation signaling pathways in skeletal muscle and liver of beef cattle with divergent residual feed intake phenotypes following an acute standardized meal.

October 17, 2023animalOpen Access

Comparative analysis of signalling pathways in tissue protein metabolism in efficient and non-efficient beef cattle: acute response to an identical single meal size

View Full Paper

Ask AI

Bookmark

Authors

PGPablo Guarnido-LopezTexas A&M University IMIsabelle Ortigues MartyUniversité Clermont Auvergne JDJ. DavidHospices Civils de Lyon

Discussion

Member takes

Implication

Comparative study shows lower protein degradation and faster muscle synthesis in feed-efficient beef cattle, indicating enhanced metabolic responsiveness to feeding.

Key Points

To determine postmortem protein synthesis and degradation signaling pathways in skeletal muscle and liver of beef cattle with divergent residual feed intake phenotypes following an acute standardized meal.
Assessed 32 Charolais young bulls with extreme residual feed intake (RFI) phenotypes fed either a high-starch/high-protein or high-fibre/low-protein concentrate diet.
Provided an identical 2.5 kg single test meal 3 hours prior to slaughter, collecting blood samples at 3 hours and liver/muscle tissue at 3.5 hours postprandially.
Quantified protein degradation (autophagy, ubiquitin) and synthesis signaling pathways using western blot analysis alongside hepatic transaminase activity assays.
Efficient RFI bulls had significantly lower muscle ubiquitin abundance (P = 0.04), tended toward lower liver ubiquitin (P = 0.08) and alanine transaminase activity (P = 0.06), and showed a positive correlation between degradation potential and amino acid catabolism (r = 0.52, P = 0.004).
Muscle protein synthesis activation was accelerated in efficient RFI bulls, evidenced by a greater phosphorylated to total ribosomal protein S6 kinase ratio (P = 0.05) across diets, while hepatic synthesis showed no significant difference (P > 0.05).
Efficient bulls showed higher postprandial plasma β-hydroxybutyrate (P < 0.05) and higher insulin concentrations on the high-starch diet compared with non-efficient bulls.

Cite This Study

Guarnido-Lopez et al. (2023) studied this question.

synapsesocial.com/papers/6a8500bf29c86ef59c8aa720 https://doi.org/10.1016/j.animal.2023.101017

View Full Paper

Ask AI

Bookmark

Implication

Comparative study shows lower protein degradation and faster muscle synthesis in feed-efficient beef cattle, indicating enhanced metabolic responsiveness to feeding.

Comparative analysis of signalling pathways in tissue protein metabolism in efficient and non-efficient beef cattle: acute response to an identical single meal size

Discussion

Member takes

Implication

Key Points

Cite This Study

Comparative analysis of signalling pathways in tissue protein metabolism in efficient and non-efficient beef cattle: acute response to an identical single meal size

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider