A Methanol-Utilizing Bacterium: Ii. Studies on the Pathway of Methanol Assimilation

Two simultaneous pathways for the fixation of carbon from one-carbon compounds by a Pseudomonas sp. (PRL-W4) are postulated. Carbonate and formate form parts of one pathway and methanol and formaldehyde the other. It is presumed that both the formate and formaldehyde are incorporated as serine by the organism. The energy necessary for the incorporation of carbonate carbon can be supplied by the oxidation of methanol or of formate but not formaldehyde. No formaldehyde dehydrogenase activity was found.