What question did this study set out to answer?

Investigate how the C-terminal region of SNAP25 and calcium regulate the interaction between synaptotagmin and SNARE complexes during exocytosis.

March 1, 2000Journal of Biological ChemistryOpen Access

The C Terminus of SNAP25 Is Essential for Ca2+-dependent Binding of Synaptotagmin to SNARE Complexes

View Full Paper

Ask AI

Bookmark

Authors

RGRoy GeronaUniversity of California, San Francisco ELEric LarsenUniversity of Massachusetts Chan Medical School JKJudith A. KowalchykDartmouth College

Discussion

Member takes

Implication

In vitro study demonstrates that the SNAP25 C-terminus mediates calcium-dependent synaptotagmin-SNARE binding in cell membranes, indicating a key mechanism for calcium-triggered exocytosis.

Key Points

Investigate how the C-terminal region of SNAP25 and calcium regulate the interaction between synaptotagmin and SNARE complexes during exocytosis.
Treated PC12 cell membranes with botulinum neurotoxins BoNT A and BoNT E to selectively cleave specific residues at the C-terminus of SNAP25.
Assayed calcium-dependent exocytosis and binding affinity of synaptotagmin to SNAP25, SNAP25-syntaxin, and SNAP25-syntaxin-VAMP complexes across varied calcium concentrations.
BoNT E cleavage completely blocked the late calcium-dependent stage of exocytosis and abolished synaptotagmin binding to SNAP25.
BoNT A cleavage partially inhibited exocytosis and shifted the calcium requirement higher, a defect reversed by elevated calcium levels.
The intact SNAP25 C-terminus was required for calcium-stimulated synaptotagmin binding to monomeric SNAP25 and assembled SNARE complexes.

Cite This Study

Gerona et al. (2000) studied this question.

synapsesocial.com/papers/6a850432e7eca461fe847bfa https://doi.org/10.1074/jbc.275.9.6328

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates that the SNAP25 C-terminus mediates calcium-dependent synaptotagmin-SNARE binding in cell membranes, indicating a key mechanism for calcium-triggered exocytosis.

The C Terminus of SNAP25 Is Essential for Ca2+-dependent Binding of Synaptotagmin to SNARE Complexes

Discussion

Member takes

Implication

Key Points

Cite This Study

The C Terminus of SNAP25 Is Essential for Ca2+-dependent Binding of Synaptotagmin to SNARE Complexes

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider