Suspensions of Pseudomonas grown on phenylacetic acid oxidized 3,4-dihydroxy-phenylacetic acid to δ-carboxymethyl-α-hydroxymuconic semialdehyde. The compound was characterized by conversion to 2,5-pyridine dicarboxylic acid. The results show that cleavage of the aromatic ring occurs between carbons 2 and 3. δ-Carboxymethyl-α-hydroxymuconic semialdehyde is relatively unstable. Maximum absorption of the compound is obtained in acid at 318 mμ and in alkali at 380 mμ. The ratio of optical densities (380/318) is 1.8. In 2% sodium carbonate solution the molar extinction coefficient at 380 mμ is 2.7 × 10 4 . Other chemical properties of the cleavage product are described.
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Blakley et al. (1967) studied this question.
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