IT now seems clear that flavin-adenine-dinucleotide acts as the coenzyme or "prosthetic group" of at least five enzymes of biological interest: (1) d-amino acid oxidase [Warburg & Christian, 1938], (2) the flavoprotein from milk of Corran & Green [1938] which is at all events similar to the xanthine oxidase of Ball [1939], (3) the yeast flavoprotein of Haas [1938], (4) diaphorase of Euler & Hellstr6m [1938], or coenzyme factor of Dewan & Green [1938] (cf. also Straub [1939]; Straub et at. [1939]), and (5) yeast fumarate hydrogenase [Fischer et al. 1939]. In view of the great importance of this nucleotide it seemed of interest to determine the amount present in boiled extracts of various rat tissues under different experimental conditions.
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Ochoa et al. (1939) studied this question.
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