What question did this study set out to answer?

To fully characterize the N-glycan, O-glycan, and glycosylphosphatidylinositol (GPI) anchor structures of human erythrocyte CD59.

March 1, 1997Journal of Biological ChemistryOpen Access

The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59

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Authors

PRPauline M. RuddUniversity College Dublin BMB. Paul MorganUniversity College Dublin MWMark R. WormaldScripps Research Institute

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Implication

Biochemical analysis reveals the complete glycan and GPI anchor structures of erythrocyte CD59, suggesting reticulocytes produce a conserved anchor type.

Key Points

To fully characterize the N-glycan, O-glycan, and glycosylphosphatidylinositol (GPI) anchor structures of human erythrocyte CD59.
Extracted and analyzed the GPI anchor core glycan and phosphatidylinositol lipid moieties from human erythrocyte CD59.
Sequenced total N- and O-glycan pools using an enzyme array digestion strategy.
Constructed a molecular model combining the newly determined glycan structures with the known NMR solution structure of the extracellular domain.
The CD59 GPI anchor contains a conserved core glycan linked to an alkylacylglycerol-phospho-myo-inositol moiety virtually identical to that of erythrocyte cholinesterase.
The N-glycan pool consists of bi-antennary complex-type structures with variable lactosamine extensions and fucose residues, while NeuNAcalpha2-3Galbeta1-3GalNAc and Galbeta1-3[NeuNAcalpha2-3]GalNAc represent the predominant O-glycans.
Molecular modeling suggests these attached glycans help orient and space CD59 on the red blood cell surface while shielding it from proteolysis.

Cite This Study

Rudd et al. (1997) studied this question.

synapsesocial.com/papers/6a850633de619cf7bcde0df7 https://doi.org/10.1074/jbc.272.11.7229

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Implication

Biochemical analysis reveals the complete glycan and GPI anchor structures of erythrocyte CD59, suggesting reticulocytes produce a conserved anchor type.

The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59

Discussion

Member takes

Implication

Key Points

Cite This Study

The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59

Discussion

Member takes

Implication

Key Points

Cite This Study

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