What question did this study set out to answer?

To determine the electrophysiological effects and cellular mechanisms of prokineticin 2 (PK2) on magnocellular and parvocellular neuronal subpopulations within the rat hypothalamic paraventricular nucleus.

January 1, 2007European Journal of NeuroscienceOpen Access

Prokineticin 2 depolarizes paraventricular nucleus magnocellular and parvocellular neurons

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To determine the electrophysiological effects and cellular mechanisms of prokineticin 2 (PK2) on magnocellular and parvocellular neuronal subpopulations within the rat hypothalamic paraventricular nucleus.
Conducted blind whole-cell patch-clamp recordings on magnocellular (MNC), parvocellular preautonomic (PA), and parvocellular neuroendocrine (NE) neurons in rat hypothalamic slices.
Assessed neuronal responses to 10 nm PK2 under voltage- and current-clamp modes in the presence of tetrodotoxin (TTX), kynurenic acid, the PK2 receptor antagonist A1MPK1, and the MAPK inhibitor PD 98059.
PK2 (10 nm) depolarized 76% of MNC neurons and increased excitatory postsynaptic potential and current frequencies, with effects eliminated by TTX and kynurenic acid.
PK2 depolarized 68% of PA and 67% of NE parvocellular neurons; these responses persisted in TTX, demonstrating a direct postsynaptic action.
Depolarizations in PA and NE neurons were concentration-dependent and were abolished by both the receptor antagonist A1MPK1 and the MAPK inhibitor PD 98059.

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Implication

Ex vivo patch-clamp study demonstrates prokineticin 2 depolarizes distinct hypothalamic paraventricular neurons in rats, indicating distinct presynaptic and direct postsynaptic mechanisms.

Cite This Study

Yuill et al. (2007) studied this question.

synapsesocial.com/papers/6a850beac8734dbdafde077a https://doi.org/10.1111/j.1460-9568.2006.05293.x

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Implication

Ex vivo patch-clamp study demonstrates prokineticin 2 depolarizes distinct hypothalamic paraventricular neurons in rats, indicating distinct presynaptic and direct postsynaptic mechanisms.

Prokineticin 2 depolarizes paraventricular nucleus magnocellular and parvocellular neurons

Key Points

Discussion

Implication

Cite This Study

Prokineticin 2 depolarizes paraventricular nucleus magnocellular and parvocellular neurons

Key Points

Discussion

Implication

Cite This Study

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