What question did this study set out to answer?

To evaluate the molecular requirements of CD4, gp120, and cell-surface heparans in mediating HIV-1 virion attachment to HeLa cells compared to T-cell lines.

May 1, 1998Journal of VirologyOpen Access

Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans

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Authors

IMIsabelle MondorCentre National de la Recherche Scientifique SUSophie UgoliniCentre National de la Recherche Scientifique QSQuentin J. SattentauMax Delbrück Center

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Implication

In vitro study reveals HIV-1 attachment to HeLa cells relies on cell surface heparans rather than CD4, indicating cell-type-specific alternative pathways for viral binding.

Key Points

To evaluate the molecular requirements of CD4, gp120, and cell-surface heparans in mediating HIV-1 virion attachment to HeLa cells compared to T-cell lines.
Measured HLA-DR+ HIV-1 virion attachment to HLA-DR- cells using anti-HLA-DR antibodies across CD4+ A3.01 T cells, CD4- A2.01 T cells, and HeLa cells with variable CD4 expression.
Treated cells and virus with monoclonal antibodies against CD4, gp120 epitopes, and adhesion molecules, as well as polyanions and heparinase enzymes to assess attachment and infection.
Virion attachment to A3.01 T cells was highly CD4 dependent, whereas attachment to HeLa cells occurred at equivalent or higher levels in the absence of CD4 and was enhanced by CD4 monoclonal antibodies.
Attachment to HeLa cells was mediated by gp120 loops (inhibited by V2, V3, and CD4i antibodies) and completely blocked by polyanions or enzymatic removal of cell-surface heparans via heparinase.
Despite CD4 being dispensable for virion attachment to HeLa cells, CD4 expression remained strictly required for subsequent cellular infection.

Cite This Study

Mondor et al. (1998) studied this question.

synapsesocial.com/papers/6a851230d170c02b45de0627 https://doi.org/10.1128/jvi.72.5.3623-3634.1998

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Implication

In vitro study reveals HIV-1 attachment to HeLa cells relies on cell surface heparans rather than CD4, indicating cell-type-specific alternative pathways for viral binding.

Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans

Discussion

Member takes

Implication

Key Points

Cite This Study

Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans

Discussion

Member takes

Implication

Key Points

Cite This Study

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