CONTAMINATION OF PURIFIED PROSTATE-SPECIFIC ANTIGEN PREPARATIONS BY KALLIKREIN hK2

No AccessJournal of UrologyInvestigative Urology1 Apr 1998CONTAMINATION OF PURIFIED PROSTATE-SPECIFIC ANTIGEN PREPARATIONS BY KALLIKREIN hK2 GILLES FRENETTE, YANNICK GERVAIS, ROLAND R. TREMBLAY, and JEAN Y. DUBE GILLES FRENETTEGILLES FRENETTE More articles by this author , YANNICK GERVAISYANNICK GERVAIS More articles by this author , ROLAND R. TREMBLAYROLAND R. TREMBLAY More articles by this author , and JEAN Y. DUBEJEAN Y. DUBE (Dube) Requests for reprints: Hormonal Bioregulation Laboratory, CHUL Research Center, 2705 Laurier Boulevard, Sainte-Foy, Quebec, Canada G1V 4G2. More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)63619-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail This paper ascertained the contamination by hK2 of two types of PSA preparation, that of Sensabaugh and Blake (J.Urol., 144: 1523, 1990) and that of Deperthes et al (J. Androl., 17: 659, 1996). In the first procedure, the free forms of hK2 co-migrated with PSA during the CM-Sephadex and the Sephacryl S-200 steps. By contrast, in the second procedure a very high proportion of hK2 was separated from PSA. In two different Sensabaugh and Blake procedures, the hK2 contamination per micro g. of PSA was found to be respectively 0.3 and 1.0 ng. We conclude that hK2 is a quantitatively minor contaminant of some PSA preparations. That contamination is probably of little consequence for PSA standardization but it could lead to erroneous conclusions in enzymatic studies of PSA. References 1 : Human prostate-specific antigen: structural and functional similarity with serine-proteases. Proc. Natl Acad. Sci. U. S. A.1986; 83: 3166. Google Scholar 2 : The chymotrypsin-like activity of human prostate-specific antigen gamma-seminoprotein. Febs Lett.1987; 225: 168. 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Google Scholar Hormonal Bioregulation Laboratory, CHUL Research Center and Laval University, Sainte-Foy, Quebec, Canada.© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 159Issue 4April 1998Page: 1375-1378 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information GILLES FRENETTE More articles by this author YANNICK GERVAIS More articles by this author ROLAND R. TREMBLAY More articles by this author JEAN Y. DUBE (Dube) Requests for reprints: Hormonal Bioregulation Laboratory, CHUL Research Center, 2705 Laurier Boulevard, Sainte-Foy, Quebec, Canada G1V 4G2. More articles by this author Expand All Advertisement PDF downloadLoading ...