Prostate Specific Antigen Releases a Kinin-Like Substance on Proteolysis of Seminal Vesicle Fluid that Stimulates Smooth Muscle Contraction

No AccessJournal of UrologyInvestigative Urology1 Feb 1996Prostate Specific Antigen Releases a Kinin-Like Substance on Proteolysis of Seminal Vesicle Fluid that Stimulates Smooth Muscle Contraction Jan Fichtner, Howard C.B. Graves, Kay Thatcher, Cheryl Yemoto, and Linda M.D. Shortlife Jan FichtnerJan Fichtner More articles by this author , Howard C.B. GravesHoward C.B. Graves More articles by this author , Kay ThatcherKay Thatcher More articles by this author , Cheryl YemotoCheryl Yemoto More articles by this author , and Linda M.D. ShortlifeLinda M.D. Shortlife More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66512-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We investigated whether purified prostate specific antigen (PSA), a seminal plasma serine protease of the kallikrein enzyme family, is capable of releasing kinin-like peptides from natural substrate glycoproteins in human seminal vesicle fluid. Materials and Methods: An in vivo rat bladder model was used to monitor for release of substances capable of inducing smooth muscle contractions. Purified PSA, seminal vesicle fluid (SVF) from radical prostatectomy specimens, bradykinin, saline and a bradykinin antagonist were injected intravesically into urethane-anesthetized rats, and the resulting bladder contractions were measured. Results: Injection of either PSA or SVF alone did not induce bladder contractions. Injection of a mixture of SVF and PSA preincubated 15 minutes, however, induced strong bladder contractions (23 plus/minus 7 cm. H2 O) that decreased with time (4 plus/minus 2 cm. H2 O, after 90 minutes). Similar contractions were observed after injection of bradykinin (10 sup -4 M. = 39 plus/minus 14, 10 sup -6 M. = 27 plus/minus 9, 10 sup -8 M. = 7 plus/minus 4 cm. H2 O). Addition of a bradykinin antagonist to the PSA-SVF mixture prior to injection blocked the observed bladder contractions (23 plus/minus 7 cm. H2 O before, versus 0.3 plus/minus 1.2 cm. H2 O after adding antagonist). 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Google Scholar Department of Urology, Stanford University Medical Center, Stanford, California.This paper was presented in part at the Eighty-Ninth Annual Meeting of the American Urological Association, May, 1994.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byFRENETTE G, GERVAIS Y, TREMBLAY R and DUBE J (2018) CONTAMINATION OF PURIFIED PROSTATE-SPECIFIC ANTIGEN PREPARATIONS BY KALLIKREIN hK2Journal of Urology, VOL. 159, NO. 4, (1375-1378), Online publication date: 1-Apr-1998. Volume 155Issue 2February 1996Page: 738-742 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Jan Fichtner More articles by this author Howard C.B. Graves More articles by this author Kay Thatcher More articles by this author Cheryl Yemoto More articles by this author Linda M.D. Shortlife More articles by this author Expand All Advertisement PDF downloadLoading ...