A glowing success in enzyme assay: The first high-throughput format for the determination of synthetic activity of lipases and esterases in organic solvents is described. Acetaldehyde released by enzyme-catalyzed esterification between a vinyl ester and an alcohol is derivatized in situ with a hydrazine 1 to give a highly fluorescent product 2. Thus, active and stable enzymes can be easily identified in a microtiter plate format.
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Konarzycka‐Bessler et al. (2003) studied this question.
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