Investigation of Blastocystis Subspecies in Water Samples Collected from Ordu Province

Objective: subspecies in water samples collected from Ordu province. Methods: gene was amplified by polymerase chain reaction (PCR). Sequence analysis was performed on the positive PCR products and the base sequences were aligned using Bioedit. Phylogeny trees were drawn and Blastocystis subspecies were identified. Results: ST-3 subspecies were found in Karabalçık River. The lowest genetic distance was found between ST-1 subspecies and the samples from Bülbül River, Kacalı River and Bolaman Stream. The nucleotide similarities between them were 98.8%, 76.6 and 98.8%, respectively. The lowest genetic distance was found between Karabalçık River and ST-3 subspecies and the nucleotide similarity between them was 99.1%. Conclusion: spp. in the surface water and drinking water samples in Ordu province of the Black Sea area in Turkey.