Electrochemical Studies of Vitamin K3 Interacting with Hemoglobin and Determination of Hemoglobin

Electrochemical investigation of the interaction of vitamin K3(VK3) with hemoglobin (Hb) on a Hg electrode is reported for the first time. With the addition of hemoglobin in vitamin K3solution, both the reduction and oxidation currents decrease but with no change of peak potentials. The reaction of vitamin K3with hemoglobin forms a kind of nonelectrochemical active supramolecular complex Hb–VK3. The equilibrium constant for complex is calculated as 3.75×104. A satisfactory result has been obtained for the determination of hemoglobin in clinical blood samples.