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Several previously undescribed ultrastructural features of chicken embryo lethal orphan (CELO) virus replication in chicken chorioallantoic membrane (CAM) have been observed. The virus particles appeared to fuse with the plasmalemma at adsorption sites. Newly synthesized virus was detected at 12–14 hours post infection (PI) by titration of infectivity in embryonating chicken eggs. These infectivity titers reached a maximum at 30 h PI. The two membranes of the nuclear envelope of infected cells separated to form a perinuclear space. CELO virus in crystalline arrays was observed in nuclei of only endodermal CAM cells. CELO virus was not observed by electron microscopy in the brain, liver or lungs of allantoic or intravenous inoculated embryos. Thus, the invasiveness of the Phelps strain of CELO virus appears restricted to the CAM.
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Slifkin et al. (1971) studied this question.
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