What question did this study set out to answer?

Determine whether pig brain diacylglycerol kinase undergoes autophosphorylation or is phosphorylated by endogenous protein kinases.

May 26, 1986FEBS Letters

Phosphorylation of pig brain diacylglycerol kinase by endogenous protein kinase

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Determine whether pig brain diacylglycerol kinase undergoes autophosphorylation or is phosphorylated by endogenous protein kinases.
Incubated partially purified pig brain diacylglycerol kinase preparations with Mg2+ and [gamma-32P]ATP.
Immunoprecipitated the kinase to evaluate serine phosphorylation across varying pH levels and in the presence of calcium and cyclic nucleotides.
Diacylglycerol kinase did not autophosphorylate, but was phosphorylated on serine residues by a copurifying endogenous protein kinase.
Phosphorylation operated independently of cyclic nucleotides and Ca2+, peaked at pH 5.5, and achieved a maximal incorporation of approximately 0.25 mol Pi per mole of kinase.

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Implication

Biochemical analysis reveals serine phosphorylation of diacylglycerol kinase by an endogenous kinase in pig brain, suggesting it can act as a phosphoprotein.

Cite This Study

Kanoh et al. (1986) studied this question.

synapsesocial.com/papers/6a85218a563dbb7b86d97ab2 https://doi.org/10.1016/0014-5793(86)80577-4

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Implication

Biochemical analysis reveals serine phosphorylation of diacylglycerol kinase by an endogenous kinase in pig brain, suggesting it can act as a phosphoprotein.

Phosphorylation of pig brain diacylglycerol kinase by endogenous protein kinase

Key Points

Discussion

Implication

Cite This Study

Phosphorylation of pig brain diacylglycerol kinase by endogenous protein kinase

Key Points

Discussion

Implication

Cite This Study

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