What question did this study set out to answer?

To determine the nature of the carbohydrate-polypeptide linkages, the adjacent amino acid sequences, and the overall composition and size of carbohydrate units in alpha 1-acid glycoprotein.

June 1, 1965Biochemical JournalOpen Access

THE CARBOHYDRATE-POLYPEPTIDE LINKAGES, THE AMINO ACID SEQUENCES OF THE PEPTIDES ADJACENT TO SOME OF THESE BONDS, AND THE COMPOSITION AND SIZE OF THE CARBOHYDRATE UNITS OF α1-ACID GLYCOPROTEIN

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Authors

MSMotoyoshi SATAKETohoku University TOT. OkuyamaHokkaido University KIKazuhíko IshiharaFujita Health University

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Implication

Biochemical analysis reveals carbohydrate-peptide linkages in alpha 1-acid glycoprotein, demonstrating that sugar units attach via aspartic acid amide groups.

Key Points

To determine the nature of the carbohydrate-polypeptide linkages, the adjacent amino acid sequences, and the overall composition and size of carbohydrate units in alpha 1-acid glycoprotein.
Fractionated glycopeptides derived from a proteolytic digest of sialic acid-free alpha 1-acid glycoprotein into five primary fractions using DEAE-cellulose column chromatography.
Determined N- and C-terminal amino acids, peptide sequences, and carbohydrate composition using periodate oxidation, hydrazinolysis, dinitrophenylation, and mild acid hydrolysis.
Determined that the glycoprotein contains five carbohydrate units, with average molecular weights of 2,200 for sialic acid-free units and 3,000 for sialic acid-containing units (one fraction reached 3,100).
Identified three main glycopeptide sequences—Asp(NH2)-Pro-Lys, Thr-Asp(NH2)-Ala, and Asp(NH2)-Gly-Thr—confirming that at least four carbohydrate units link directly to the beta-carboxyl amide group of aspartic acid.
Identified structural heterogeneity in carbohydrate unit size and in fucose and sialic acid content, showing fucose is not linked to sialic acid and possesses higher stability against acid hydrolysis.

Cite This Study

Satake et al. (1965) studied this question.

synapsesocial.com/papers/6a85284e69a073149ac6adbb https://doi.org/10.1042/bj0950749

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Implication

Biochemical analysis reveals carbohydrate-peptide linkages in alpha 1-acid glycoprotein, demonstrating that sugar units attach via aspartic acid amide groups.

THE CARBOHYDRATE-POLYPEPTIDE LINKAGES, THE AMINO ACID SEQUENCES OF THE PEPTIDES ADJACENT TO SOME OF THESE BONDS, AND THE COMPOSITION AND SIZE OF THE CARBOHYDRATE UNITS OF α1-ACID GLYCOPROTEIN

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Key Points

Cite This Study

THE CARBOHYDRATE-POLYPEPTIDE LINKAGES, THE AMINO ACID SEQUENCES OF THE PEPTIDES ADJACENT TO SOME OF THESE BONDS, AND THE COMPOSITION AND SIZE OF THE CARBOHYDRATE UNITS OF α1-ACID GLYCOPROTEIN

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Implication

Key Points

Cite This Study

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