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A simple and highly reproducible method was developed for the dissociation of ovine luteal tissue into isolated cell suspensions using a crude collagenase preparation in Medium 199. The procedure required less than 3 hr. The mean yield of cells was 81.8 ± 9.1 × 106 cells/g tissue. The resulting cell preparation synthesized progesterone and responded to the addition of LH or HCG, but not prolactin, with increased progesterone production. The quantity of progesterone produced was related to the level of LH added. The cells were still steroidogenically capable and responsive to LH after storage for 48 hr at 4°.
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Simmons et al. (1976) studied this question.
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