The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1. 1Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system1978 · 57 citations
2. 2Preparation of fully active ficin from <i>Ficus glabrata</i> by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe1976 · 60 citations
3. 34-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain1976 · 31 citations
4. 4Yeast hexokinases. VII. Essential and nonessential thiols of yeast hexokinase. Reactions with iodoacetate and iodoacetamide1975 · 29 citations
5. 5Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols1977 · 26 citations