Monogalactosyl and digalactosyl diglycerides were separated from leaf and Chlorella vulgaris lipid extracts by thin-layer chromatography with Silica Gel G as the stationary phase and acetone-acetic acid-water as the mobile phase. Phospholipids are completely removed and with two-dimensional development can themselves be fractionated.
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Harold W. Gardner (1968) studied this question.
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