What question did this study set out to answer?

To elucidate the distinct and synergistic cellular mechanisms by which ARBs and SGLT2 inhibitors confer renoprotection in diabetic kidney disease.

October 21, 2021Molecular TherapyOpen Access

Kidney single-cell transcriptome profile reveals distinct response of proximal tubule cells to SGLT2i and ARB treatment in diabetic mice

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Authors

JWJinshan WuThe Affiliated Yongchuan Hospital of Chongqing Medical University ZSZeguo SunHarbin Medical University SYShumin YangThe Affiliated Yongchuan Hospital of Chongqing Medical University

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Implication

Single-cell transcriptomic profiling reveals distinct proximal tubule responses to SGLT2i and ARB therapies in diabetic mice, highlighting complementary pathways of renoprotection.

Key Points

To elucidate the distinct and synergistic cellular mechanisms by which ARBs and SGLT2 inhibitors confer renoprotection in diabetic kidney disease.
Conducted single-cell RNA sequencing on kidney cells from diabetic db/db mice treated with vehicle, ARBs, SGLT2i, or combination therapy, alongside non-diabetic db/m control mice.
Identified and characterized kidney cell subclusters, with subsequent validation using immunostaining in both diabetic mouse models and human kidney disease specimens.
Identified 10 distinct kidney cell clusters, showing that ARBs primarily downregulate inflammatory and fibrotic pathways, whereas SGLT2i predominantly modulates mitochondrial function in proximal tubular cells.
Discovered an expanded proximal tubule subcluster specific to diabetic kidney disease that was reversed by treatment and confirmed in both mouse and human diabetic kidneys.

Cite This Study

Wu et al. (2021) studied this question.

synapsesocial.com/papers/6a8548c2c2b9aa95f6f23d76 https://doi.org/10.1016/j.ymthe.2021.10.013

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Implication

Single-cell transcriptomic profiling reveals distinct proximal tubule responses to SGLT2i and ARB therapies in diabetic mice, highlighting complementary pathways of renoprotection.

Kidney single-cell transcriptome profile reveals distinct response of proximal tubule cells to SGLT2i and ARB treatment in diabetic mice

Discussion

Member takes

Implication

Key Points

Cite This Study

Kidney single-cell transcriptome profile reveals distinct response of proximal tubule cells to SGLT2i and ARB treatment in diabetic mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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