Rhinovirus Replication in Suspension Cultures of HeLa Cells

Rhinovirus-infected HeLa suspension cultures did not increase in cell number and lost viability late in the infectious cycle. Maximum adsorption occurred within 30 min with approximately 40% of the virus being adsorbed. Yields of intracellular virus of about 8-10 PFU/cell were obtained at 12 hr after infection. Comparable levels of extracellular virus were not obtained until 30 hr after infection. The suspended cell culture system should have practical value in studies on replication and for obtaining concentrated preparations of virions.