What question did this study set out to answer?

To determine whether ubiquitin conjugation and ATP hydrolysis are necessary for the proteasomal degradation of oxidatively damaged proteins in intact cells.

December 28, 2002Journal of Biological ChemistryOpen Access

Ubiquitin Conjugation Is Not Required for the Degradation of Oxidized Proteins by Proteasome

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Authors

RSReshma ShringarpureSpero Therapeutics (United States)TGTilman GrunePreventive Cardiology JMJana MehlhaseUniversity of Southern California

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Implication

Experimental study demonstrates ubiquitin-independent degradation of oxidized proteins in intact cells, indicating direct proteasomal clearance without ATP.

Key Points

To determine whether ubiquitin conjugation and ATP hydrolysis are necessary for the proteasomal degradation of oxidatively damaged proteins in intact cells.
Measured intracellular degradation of oxidized proteins in cells with compromised ubiquitin-conjugating activity treated with or without proteasome inhibitors.
Assessed ubiquitinylation levels and proteasomal degradation rates of progressively oxidized model proteins, including lysozyme and ferritin.
Evaluated the effect of exogenous ATP supplementation on the degradation rates of oxidized proteins in cell lysates.
Cells with compromised ubiquitin-conjugating activity still preferentially degraded oxidized intracellular proteins at near-normal rates, and this degradation was inhibited by proteasome inhibitors.
Progressive oxidation of lysozyme and ferritin did not increase their ubiquitinylation, yet both oxidized forms were preferentially degraded by the proteasome.
Addition of ATP to cell lysates did not significantly alter the degradation rates of oxidized proteins, excluding an ATP requirement for this clearance pathway.

Cite This Study

Shringarpure et al. (2002) studied this question.

synapsesocial.com/papers/6a854fe995de83b44dd593b5 https://doi.org/10.1074/jbc.m206279200

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Implication

Experimental study demonstrates ubiquitin-independent degradation of oxidized proteins in intact cells, indicating direct proteasomal clearance without ATP.

Ubiquitin Conjugation Is Not Required for the Degradation of Oxidized Proteins by Proteasome

Discussion

Member takes

Implication

Key Points

Cite This Study

Ubiquitin Conjugation Is Not Required for the Degradation of Oxidized Proteins by Proteasome

Discussion

Member takes

Implication

Key Points

Cite This Study

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