13C NMR Study of Conformation and Mobility of 145-Base-Pair Poly(dA-dT) . poly(dA-dT) in Solution

Natural abundance of 13C NMR spectra were obtained for 145-base-pair poly(dA-dT) · poly(dA-dT) in solution. Two confermers of deoxyribose are present in poly(dA-dT) · poly(dA-dT) in equal amounts, suggesting that the alternating conformation of the phosphodiester backbone previously demonstrated by 31P NMR extends to the deoxyribose residues. The local mobilities of bases and sugars are different for thymidine and adenosine residues.