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Sorghum plants were allowed to assimilate known relative amounts of 14C in the two periods: (i) from full expansion of the third uppermost leaf to anthesis; and (ii) from anthesis to grain maturity. By comparing relative 14C activity in the mature grain, the relative partitioning of photosynthate between grain production and other uses in the two periods was calculated. Plant and grain dry weight data from another experiment showed the amounts of net photosynthesis in the two periods. It was then possible to calculate the maximum amount of the material assimilated in the pre-anthesis period that could have gone to grain production, and thus the percentage of grain material derived from this earlier period. The estimate was 12%.
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Fischer et al. (1971) studied this question.
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