PulseTrendingJournal ClubResearchersJournalsExplore
Instagram
HomeTrendingJournal ClubExplore
Synapse
⌘+K
Synapse
August 19, 2026New PhytologistOpen Access

The combination of morphogenic regulators BABY BOOM and GRF ‐ GIF improves maize transformation efficiency and promotes leaf regeneration

View Full Paper
Ask AI
Bookmark
Share

Authors

ZCZongliang ChenJZJiaqi ZhouMGMary Galli

Discussion

Loading...

Member takes

Overview

Experimental study demonstrates enhanced transformation efficiency and leaf regeneration in maize via combined morphogenic regulators, indicating improved methods for crop genetic engineering.

Key Points

  • Develop an efficient, accessible maize transformation and regeneration system by combining the morphogenic regulators BABY BOOM and GRF4-GIF1.
  • Co-expressed the wheat GRF4-GIF1 chimera and maize BABY BOOM (BBM) transcription factor in immature embryos across B104 and other maize lines using a modified QuickCorn protocol.
  • Tested the system's compatibility with CRISPR-Cas9 genome editing, assessed transgenic pollen transmission, and conducted RNA-seq profiling on regenerating leaf cultures.
  • The combined GGB system produced a 7-fold higher transformation efficiency compared to either morphogenic regulator alone, regenerating plantlets in approximately 2 months without requiring transgene excision.
  • Transgenic pollen transmission was significantly reduced, and media supplementation with asparagine and trehalose—identified through RNA-seq—significantly enhanced leaf regeneration via somatic embryogenesis.

Cite This Study

Chen et al. (2026) studied this question.

synapsesocial.com/papers/6a85637603308d306e2d6955https://doi.org/10.1111/nph.71508
View Full Paper
Ask AI
Bookmark
Share