Experimental imaging study demonstrates hybrid iterative ptychographic microscopy achieves 60-nm resolution without depletion lasers, highlighting broad utility across advanced optical modalities.
By utilizing an array detector to replace the single-element detector in confocal microscopy, Image Scanning Microscopy (ISM) overcomes the inherent trade-off between resolution and signal-to-noise ratio (SNR), thereby enhancing its ability to resolve sub-diffraction features. However, practical ISM performance often falls short of the theoretical ideal, leaving scope for further advancement. Here, we present hybrid iterative ptychographic super-resolution microscopy (HiM), which integrates a honeycomb array detection architecture with a hybrid high-frequency extension algorithm to achieve a 1.5-fold increase in detection efficiency and a spatial resolution of ~ 60 nm. Experiments demonstrate that HiM outperforms comparative algorithms, achieving a STED-like apparent resolution even in the absence of a depletion laser. The verified compatibility with modalities such as stimulated emission depletion microscopy (STED) and fluorescence lifetime imaging microscopy (FLIM) confirms the practical utility of HiM for various imaging requirements.
No takes yet. Share an insight, caveat, or question.
Huang et al. (2026) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: