Systematic review analyzes reconstruction algorithms to improve image quality in multifocal image scanning microscopy, suggesting new methods for researchers.
Key Points
This review aims to summarize recent advancements in reconstruction algorithms for multifocal image scanning microscopy (MISM) and address reconstruction challenges.
Systematic review of current reconstruction techniques in MISM
Analysis of physical implementation and challenges during image reconstruction
Development of a structured workflow including preprocessing, calibration, and reconstruction methods.
Outlined major challenges in MISM related to inter-pinhole crosstalk and image degradation.
Described a structured workflow that enhances reconstruction through various algorithms.
Provided an outlook on future developments in MISM reconstruction.