What question did this study set out to answer?

To determine how TGF-β1 signaling in fibro-adipogenic progenitors drives muscle fibrosis after ischemia-reperfusion injury and to evaluate whether TGF-β neutralization restores regenerative capacity.

August 19, 2026The FASEB Journal

Targeting TGF ‐β to Attenuate Ischemia–Reperfusion Associated Muscle Fibrosis and Enhance Regeneration: A Preclinical Study

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Authors

HMHongru MaNanjing Drum Tower Hospital ZZZhenjia ZhongVrije Universiteit Amsterdam HZHeng ZhengZhejiang Hospital

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Implication

Preclinical animal study demonstrates TGF-beta neutralization reduces fibrosis and restores myofiber area in injured skeletal muscle, indicating a therapeutic target for trauma recovery.

Key Points

To determine how TGF-β1 signaling in fibro-adipogenic progenitors drives muscle fibrosis after ischemia-reperfusion injury and to evaluate whether TGF-β neutralization restores regenerative capacity.
Induced acute skeletal muscle injury via cardiotoxin injection into the tibialis anterior of 8-week-old male C57BL6 mice, followed by transient femoral vessel clamping at 3 days to model ischemia-reperfusion.
Administered TGF-β neutralizing antibodies in vivo to test therapeutic efficacy against ischemia-reperfusion injury.
Quantified myofiber cross-sectional area, fibrotic area, fibro-adipogenic progenitors, and expression of Collagen I, Collagen III, and TGF-β1 using histology, flow cytometry, western blotting, and immunostaining.
Ischemia-reperfusion injury resulted in smaller myofiber cross-sectional area, increased fibrotic tissue, and elevated protein expression of Collagen I, Collagen III, and TGF-β1 compared to injury alone.
TGF-β1 directly stimulated Collagen I expression within isolated fibro-adipogenic progenitors.
In vivo treatment with TGF-β neutralizing antibodies significantly attenuated skeletal muscle fibrosis and enhanced myofiber cross-sectional area and regeneration.

Cite This Study

Ma et al. (2026) studied this question.

synapsesocial.com/papers/6a8563ae03308d306e2d702d https://doi.org/10.1096/fj.202601893r

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Implication

Preclinical animal study demonstrates TGF-beta neutralization reduces fibrosis and restores myofiber area in injured skeletal muscle, indicating a therapeutic target for trauma recovery.

Targeting TGF ‐β to Attenuate Ischemia–Reperfusion Associated Muscle Fibrosis and Enhance Regeneration: A Preclinical Study

Discussion

Member takes

Implication

Key Points

Cite This Study

Targeting TGF ‐β to Attenuate Ischemia–Reperfusion Associated Muscle Fibrosis and Enhance Regeneration: A Preclinical Study

Discussion

Member takes

Implication

Key Points

Cite This Study

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