Simultaneous quantification of seventeen kratom alkaloids and metabolites in human plasma and its application to clinical pharmacokinetic sample analysis

Siva Rama Raju Kanumuri; Manoj Gundeti; Sushobhan Mukhopadhyay; Naftali Z. Zeilinger; C. Austin Zamarripa; Kirsten E. Smith; Christopher R. McCurdy; Abhisheak Sharma

doi:10.1080/13880209.2026.2715806

August 17, 2026Pharmaceutical BiologyOpen Access

Simultaneous quantification of seventeen kratom alkaloids and metabolites in human plasma and its application to clinical pharmacokinetic sample analysis

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Key Points

To develop and validate an ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) assay for the simultaneous quantification of 12 kratom alkaloids and 5 mitragynine metabolites in human plasma.
Developed and validated an FDA-compliant UPLC-MS/MS method using protein precipitation extraction and stabilization protocols for unstable metabolites (7-hydroxymitragynine and 9-hydroxycorynantheidine).
Applied the assay to human plasma samples from a clinical trial involving regular kratom users (N=4) following oral administration.
The bioanalytical method demonstrated linearity across a concentration range of 1 to 250 ng/mL.
Most kratom alkaloids underwent rapid absorption after oral administration, reaching peak plasma concentrations within 1 to 2 hours post-dose.
Mitragynine, speciociliatine, mitraciliatine, isopaynantheine, and paynantheine were identified as primary circulating alkaloids, with mitragynine 16-carboxylic acid and 9-hydroxycorynantheidine being the predominant metabolites.

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Implication

Clinical pharmacokinetic analysis reveals rapid absorption of multiple kratom alkaloids in human plasma, highlighting key circulating active compounds and metabolites.

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Kanumuri et al. (2026) studied this question.

synapsesocial.com/papers/6a8563d703308d306e2d72d2 https://doi.org/10.1080/13880209.2026.2715806

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Simultaneous quantification of seventeen kratom alkaloids and metabolites in human plasma and its application to clinical pharmacokinetic sample analysis

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Simultaneous quantification of seventeen kratom alkaloids and metabolites in human plasma and its application to clinical pharmacokinetic sample analysis

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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