What question did this study set out to answer?

To isolate, characterize, and confirm the fungal pathogen responsible for leaf spot symptoms on upland cotton in Southern Punjab.

August 19, 2026Plant Disease

First report of Alternaria alternata causing Alternaria leaf spot of cotton in Pakistan

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To isolate, characterize, and confirm the fungal pathogen responsible for leaf spot symptoms on upland cotton in Southern Punjab.
Collected 40 symptomatic upland cotton leaf samples in Multan and Rahimyar Khan districts and isolated 28 single-spored fungal cultures on potato dextrose agar.
Identified isolates through morphological characterization and molecular sequencing of ITS rDNA and GAPDH gene regions.
Performed attached leaf spray inoculation assays on 36 leaves across 12 40-day-old upland cotton plants (with n=3 untreated controls) to fulfill Koch's postulates.
Field disease incidence ranged from 20% to 25%, characterized by necrotic dark-brown concentric leaf rings reaching up to 1.5 cm.
BLASTn analysis of sequenced ITS rDNA and GAPDH gene regions demonstrated 99% to 100% sequence identity with Alternaria alternata.
Inoculated cotton plants developed concentric ring lesions after 11 to 12 days while controls remained asymptomatic, with re-isolated fungi matching the original pathogen.

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Implication

Plant pathology study reveals Alternaria alternata as the causal agent of leaf spot in upland cotton, highlighting an emerging threat to crop production.

Cite This Study

Iqbal et al. (2026) studied this question.

synapsesocial.com/papers/6a85643903308d306e2d7ce4 https://doi.org/10.1094/pdis-09-25-1833-pdn

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Implication

Plant pathology study reveals Alternaria alternata as the causal agent of leaf spot in upland cotton, highlighting an emerging threat to crop production.

First report of Alternaria alternata causing Alternaria leaf spot of cotton in Pakistan

Key Points

Discussion

Implication

Cite This Study

First report of Alternaria alternata causing Alternaria leaf spot of cotton in Pakistan

Key Points

Discussion

Implication

Cite This Study

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