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By radioimmunoelectrophoresis, antibodies associated with γ1, γ2 and γM globulins and 2 unidentified components, U1 and U2, were detected in rat antisera to BSA and BGG. When rats were immunized with these antigens adsorbed to bentonite or included in complete Freund's adjuvant, the sequence of the immune response in rats involves first the formation of γ1 antibody followed upon further immunization by γ2 antibody. Generally, γM and the unknown components, U1 and U2, only appeared in hyperimmune sera.
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Banovitz et al. (1967) studied this question.
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