What question did this study set out to answer?

To investigate whether altering the phosphorylation status and cellular localization of FoxO1 via PP2A phosphatase inhibition impairs regulatory T cell function.

March 7, 2017PLoS ONEOpen Access

Ex vivo modulation of the Foxo1 phosphorylation state does not lead to dysfunction of T regulatory cells

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To investigate whether altering the phosphorylation status and cellular localization of FoxO1 via PP2A phosphatase inhibition impairs regulatory T cell function.
Assessed Treg recruitment, airway IL-10 levels, and PP2A expression in a mouse model of house dust mite-induced allergic airway inflammation.
Treated murine Treg cells ex vivo with the PP2A inhibitor okadaic acid to evaluate FoxO1 phosphorylation, nuclear localization, differentiation, and suppressive activity.
Mice with house dust mite-induced inflammation exhibited increased lung and lymph node Treg recruitment without elevated airway IL-10, alongside decreased PP2A expression in mediastinal lymph nodes.
Okadaic acid treatment successfully increased FoxO1 phosphorylation and decreased its nuclear localization in Treg cells.
FoxO1 phosphorylation and displacement did not impair Treg differentiation, induce pro-inflammatory traits, or reduce ex vivo suppressive capability.

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Implication

Preclinical study reveals that altering FoxO1 phosphorylation via PP2A inhibition preserves regulatory T cell function ex vivo, suggesting standard assays may not fully reflect in vivo phenotypes.

Cite This Study

Penberthy et al. (2017) studied this question.

synapsesocial.com/papers/6a85655102d378e0d14e258f https://doi.org/10.1371/journal.pone.0173386

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Implication

Preclinical study reveals that altering FoxO1 phosphorylation via PP2A inhibition preserves regulatory T cell function ex vivo, suggesting standard assays may not fully reflect in vivo phenotypes.

Ex vivo modulation of the Foxo1 phosphorylation state does not lead to dysfunction of T regulatory cells

Key Points

Discussion

Implication

Cite This Study

Ex vivo modulation of the Foxo1 phosphorylation state does not lead to dysfunction of T regulatory cells

Key Points

Discussion

Implication

Cite This Study

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