In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea

In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories.In this work we validated 11 in-house real-time polymerase chain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni,