What question did this study set out to answer?

To identify the intracellular signal transduction pathway by which fibroblast growth factor-1 (FGF-1) stimulates promatrilysin expression in prostate cancer cells.

January 1, 2002NeoplasiaOpen Access

Fibroblast Growth Factor-1 Induced Promatrilysin Expression Through the Activation of Extracellular-regulated Kinases and STAT3

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To identify the intracellular signal transduction pathway by which fibroblast growth factor-1 (FGF-1) stimulates promatrilysin expression in prostate cancer cells.
Treated human LNCaP prostate cancer cells with FGF-1 in the presence or absence of the MEK1/2 pharmacological inhibitor PD98059.
Conducted transient transfections using a human matrilysin promoter-luciferase reporter construct alongside a dominant-negative STAT3 expression vector.
Assessed ERK1/2 kinase activation kinetics over 24 hours and evaluated serine phosphorylation of STAT3.
FGF-1 treatment produced sustained ERK1 and ERK2 activation up to 24 hours, whereas treatment with the MEK1/2 inhibitor PD98059 completely abolished ERK activation and promatrilysin induction.
FGF-1 induced a 4- to 5-fold increase in matrilysin promoter-driven luciferase activity, which was suppressed by MEK1/2 inhibition.
FGF-1 stimulated Ser-727 phosphorylation of STAT3 that was blocked by 20 µM PD98059, while dominant-negative STAT3 prevented matrilysin promoter transactivation.

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Implication

In vitro study reveals that fibroblast growth factor-1 promotes promatrilysin expression through ERK and STAT3 activation in prostate cancer cells, highlighting key mechanisms driving tumor invasion.

Cite This Study

Udayakumar et al. (2002) studied this question.

synapsesocial.com/papers/6a85684402a1bc3ea31e590d https://doi.org/10.1038/sj.neo.7900207

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Implication

In vitro study reveals that fibroblast growth factor-1 promotes promatrilysin expression through ERK and STAT3 activation in prostate cancer cells, highlighting key mechanisms driving tumor invasion.

Fibroblast Growth Factor-1 Induced Promatrilysin Expression Through the Activation of Extracellular-regulated Kinases and STAT3

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Discussion

Implication

Cite This Study

Fibroblast Growth Factor-1 Induced Promatrilysin Expression Through the Activation of Extracellular-regulated Kinases and STAT3

Key Points

Discussion

Implication

Cite This Study

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