Microfluidic biosensor chips that are functionalized with cysteine derivatives are readily modified with peptides and proteins by pulsed native chemical ligation. The chemoselectivity ensures a homogeneous presentation of the biomolecule at the surface, and the ligand density can be tuned in a highly programmable way. The modified surfaces are selectively responsive towards complementary analytes, thus allowing accurate kinetic and thermodynamic information to be obtained.
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Helms et al. (2007) studied this question.
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