Methods of auxin assay using the Avena coleoptile are discussed. A review is given of experimental procedure and evaluation of results in the straight-growth method using isolated sections of coleoptiles in test solutions. Possible sources of variation in the straight-growth method are investigated and discussed. A revised experimental procedure for the straight-growth method is described. The author wishes to thank Professor E. Ashby for his advice and encouragement during the course of the experiments. Thanks are also due to Mr. D. Payne, a technical assistant in the Botany Department, for his assistance in some of the assays.
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J. A. BENTLEY (1950) studied this question.
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