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A method of double isotope derivative assay of aldosterone, corticosterone, and cortisol is described. It involves acetylation with acetic- 3 H anhydride of high specific activity followed by the purification of the steroids through four paper chromatographies and the formation of a derivative by chromium trioxide oxidation. The method is particularly suited to the quantitative measurement of these steroids in the incubation media of either rat or beef adrenal slices.
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Stachenko et al. (1964) studied this question.
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