Sex and kidney ACE2 expression in primary focal segmental glomerulosclerosis: A NEPTUNE study

Figures

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  Fig 1. Tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression in subjects with focal segmental glomerulosclerosis (FSGS). Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) in male and female subjects with FSGS. Values are the mean ± SD (grey lines). Significance was defined as a P value of less than 0.05.

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  Fig 2. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with age in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with age in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 3. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with estimated glomerular filtration rate (eGFR) in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with eGFR in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 4. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with urine protein to creatine ratio (UPCR) in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with centrally measured timed UPCR in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 5. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with systolic and diastolic blood pressure in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with sitting systolic blood pressure in (A) male and (B) female subjects with FSGS. (C, D) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with sitting diastolic blood pressure in (C) male and (D) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 6. Comparison of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression in subjects with focal segmental glomerulosclerosis (FSGS) on or off RAAS blocker drugs. (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) in (A) male and (B) female subjects with FSGS who were either on or not taking RAAS blockade drugs prior to the baseline visit. Values are the mean ± SD (grey lines). Significance was defined as a P value of less than 0.05.

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  Fig 7. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with structural measurements in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with interstitial fibrosis percentage (IF %) in (A) male and (B) female subjects with FSGS. (C, D) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with tubular atrophy percentage (TA %) in (C) male and (D) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 8. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with mRNA expression of inflammation genes in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with tumor necrosis factor (TNF) expression in (A) male and (B) female subjects with FSGS. (C, D) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with monocyte chemoattractant protein 1 (MCP-1 or CCL2) expression in (C) male and (D) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 9. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with transforming growth factor beta (TGFB) mRNA expression in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with TGFB1 in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 10. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with collagen, type I, alpha 1 (COL1A1) mRNA expression in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with COL1A1 in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.

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  Fig 11. Correlation of tubular angiotensin-converting enzyme 2 (ACE2) mRNA expression with alpha smooth muscle actin (ACTA2) expression in subjects with focal segmental glomerulosclerosis (FSGS). (A, B) Tubular ACE2 mRNA expression (median-centered log2 expression by microarray analysis) correlated with ACTA2 in (A) male and (B) female subjects with FSGS. Pearson’s correlation coefficient (r) with two-tailed P values were calculated. Linear regression was used to generate the line of best fit (solid lines) with 95% confidence intervals (dotted lines). Significance was defined as a P value of less than 0.05.