Key result
Electron tomography of isometrically contracting insect flight muscle revealed that strong binding myosin attachments are found on only four F-actin subunits (the 'target zone') and exhibit an axial lever arm range of 77 degrees.
Population
Isometrically contracting insect flight muscle (cryofixed, freeze substituted, and thin sectioned)
Design
Preclinical
Authors
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Provides structural constraints on myosin attachments in isometric contraction; extends cross-bridge models but leaves open translation to mammalian cardiac muscle.
This high-resolution electron tomography study provides a structural model for the weak to strong transition in the myosin ATPase cycle during isometric contraction, highlighting azimuthal movements of the motor domain on actin.
Wu et al. (2010) studied Isometric muscle contraction. Electron tomography of cryofixed isometrically contracting insect flight muscle was evaluated on Actin-myosin attachments and lever arm orientations. Electron tomography of isometrically contracting insect flight muscle revealed that strong binding myosin attachments are found on only four F-actin subunits (the 'target zone') and exhibit an axial lever arm range of 77 degrees.
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