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Folding helpers: Coexpression of Streptomyces coelicolor chaperonins GroEL1, GroEL2 and GroES with an actinomycete-derived polyketide synthase multienzyme in Escherichia coli has beneficial effects on yield, folding and specific activity of the purified enzyme. The results strongly suggest the utility of chaperones derived from polyketide-producing actinomycete bacteria in optimising the recombinant production of PKS proteins in E. coli for detailed studies of structure and function.
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Betancor et al. (2008) studied this question.
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