Utilization of 2′-Deoxy-6-thioguanosine 5′-Triphosphate In DNA Synthesis Catalyzed by DNA Polymerase I Klenow Fragment of Escherichia Coli

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The substrate kinetics of 2′-deoxy-6-thioguanosine 5′-triphosphate for DNA polymerase I (Klenow fragment) of Escherichia coli were investigated. The analog nucleotide was readily incorporated in place of dGTP and it was misincorporated in place of dATP at low but detectable frequency.