What question did this study set out to answer?

To determine how the Wilms' tumor suppressor protein WT1 regulates transcription and to identify the specific domains responsible for transcriptional activation versus repression.

May 1, 1993Journal of Biological ChemistryOpen Access

The Wilms' tumor gene product WT1 activates or suppresses transcription through separate functional domains

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Authors

ZWZ.Y. WangPeking University QQQuan QiuPeking University TDThomas F. DeuelScripps Research Institute

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Implication

In vitro study reveals dual transcriptional activation and repression by Wilms tumor protein WT1 in cell models, indicating target-dependent gene regulation.

Key Points

To determine how the Wilms' tumor suppressor protein WT1 regulates transcription and to identify the specific domains responsible for transcriptional activation versus repression.
Engineered chimeric reporter plasmids containing varying combinations of 5' and 3' WT1-binding motifs relative to the transcription start site.
Generated wt1 truncation mutants to map the specific amino acid sequences that mediate transcriptional repression versus activation.
WT1 requires the presence of both 5' and 3' flanking binding sites to repress transcription, whereas binding to either site alone converts WT1 into a transcriptional activator.
Amino acid residues 84-179 are required for transcriptional repression, whereas amino acid residues 180-294 harbor an independent domain that drives transcriptional activation.

Cite This Study

Wang et al. (1993) studied this question.

synapsesocial.com/papers/6a85a1c635bc0eada8d5a94f https://doi.org/10.1016/s0021-9258(18)98329-8

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Implication

In vitro study reveals dual transcriptional activation and repression by Wilms tumor protein WT1 in cell models, indicating target-dependent gene regulation.

The Wilms' tumor gene product WT1 activates or suppresses transcription through separate functional domains

Discussion

Member takes

Implication

Key Points

Cite This Study

The Wilms' tumor gene product WT1 activates or suppresses transcription through separate functional domains

Discussion

Member takes

Implication

Key Points

Cite This Study

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