What question did this study set out to answer?

Isolate and characterize an alfalfa cDNA encoding a protein homologous to mammalian ERp72 and evaluate its expression across plant tissues and stress conditions.

January 1, 1992The Plant JournalOpen Access

Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose‐regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase

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Authors

BSBasil S. ShorroshKansas State University RDRichard A. DixonUniversity of North Texas

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Implication

Experimental study reveals an ERp72-like protein induced by tunicamycin in alfalfa cells, indicating a conserved role in plant endoplasmic reticulum stress responses.

Key Points

Isolate and characterize an alfalfa cDNA encoding a protein homologous to mammalian ERp72 and evaluate its expression across plant tissues and stress conditions.
Screened an alfalfa (Medicago sativa L.) cDNA library using human protein disulphide isomerase cDNA probes.
Assessed transcript levels across major alfalfa organs and in cell suspension cultures challenged with tunicamycin, calcium ionophore, heat shock, or fungal elicitor.
Identified cDNA clone G1, which encodes a 364-amino-acid polypeptide featuring a putative secretory signal sequence and two catalytic sites identical to mammalian PDI and ERp72.
Demonstrated constitutive expression of the G1 gene family across all major plant organs.
Observed marked transcriptional induction of G1 and PDI-like clone B2 following tunicamycin exposure, whereas calcium ionophore, heat shock, and fungal elicitors caused no induction.

Cite This Study

Shorrosh et al. (1992) studied this question.

synapsesocial.com/papers/6a85ac8f581d4f62e2d5b3af https://doi.org/10.1046/j.1365-313x.1992.t01-50-00999.x

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Implication

Experimental study reveals an ERp72-like protein induced by tunicamycin in alfalfa cells, indicating a conserved role in plant endoplasmic reticulum stress responses.

Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose‐regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose‐regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase

Discussion

Member takes

Implication

Key Points

Cite This Study

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